Assessing rapid viral enumeration/detection systems

By Dr. Ray Nims

In a previous posting, we alluded to the recent availability of rapid methods for identification of viruses. These technologies, together with rapid methods for enumerating viruses, should greatly expedite the quantification and identification of viruses (and bacteriophage) as compared with the existing cell culture-based approaches.

Rapid enumeration technologies are intended to replace the cell-based infectivity endpoints such as plaque assays or tissue-culture infectious dose assays, which typically require 7-10 days for completion. The use of the rapid methods may be appropriate in cases where it is not necessary to determine the infectious titer of a virus stock. An example of this might be for monitoring the amplification of viruses for preparation of live or subunit vaccines. The particle enumeration technologies include those that specifically measure viral particles and those that measure particles in general. As shown in Table 1, the particle enumeration technologies are not specific to any given virus. These are not generally useful, therefore, for viral identification, although the particle detection method associated with the NanoSight system does allow for sizing of the particles. Viral particle size is a key attribute to be aware of when, for instance, attempting to identify an unknown viral contaminant.

Table 1. Characteristics of rapid viral enumeration/identification technologies

The quantitative polymerase chain reaction (Q-PCR) and universal biosensor (Ibis T5000) technologies represent approaches that are capable of providing information both on the relative quantity of a virus in a sample and its identity. The important difference between the two is that in the former case (Q-PCR), the user is typically evaluating the identity and or quantity of a virus which is reactive with the specific primers and probes used in the assay. From an identification standpoint then, the Q-PCR technique has typically been used to confirm whether an unknown virus is related to the virus for which the assay primers and probes was designed. The degree of relatedness required is determined by the specific primers and probes used in the assay, and may be either to the genus level or the species level. Efforts are being made to incorporate primers for more highly conserved sequences to allow for more broad coverage in Q-PCR assays intended for viral screening. In the case of the universal biosensor (Ibis T5000), an unknown virus in a sample may be simultaneously identified and quantified, as long as the virus is or is closely related to one for which mass spectrometry information is present in the software used for assay analysis. Quantification in either case is in genomic units, and as with the particle enumeration methods, the readout of the quantitative nucleic acid methods does not indicate whether the virus detected is infectious. An additional nucleic acid-based method that may prove useful, in cases where relatively rapid identification of an unknown viral contaminant is needed, is deep (massively parallel) sequencing. This method is more labor intensive (and perhaps costly) then the other quantitative nucleic acid methods described above, but has the advantage that it can provide information regarding the completeness (partial vs. full-length) of the viral genomic sequences detected. This approach has displayed utility in identifying a novel picornavirus in harbor seal samples, porcine circovirus in rotavirus vaccines, and a new parvovirus in bovine serum.

Microarray screening is a technology that may be used to rapidly identify (but not enumerate) an unknown virus in a sample, provided that a probe for the virus is part of the microarray chip. Some microarray chips intended for viral identification also contain probes for conserved viral genomic sequences. In this case, the microarray may identify a novel unknown virus, at least to the genus level. As with the other rapid methods that are based on presence of specific genomic material, the assay cannot discriminate between infectious and non-infectious virus.

See Table 1 for some of the important characteristics and limitations of each method. The use of the rapid methods discussed above and in Table 1 should reduce the time needed for viral quantitation from weeks to hours, and for identification of an unknown contaminant in a sample from months (or years) to one or more days. This should greatly facilitate the monitoring of viral proliferation in manufacturing processes and the investigation of viral contamination events.

Cell Culturists….Are your human cells authenticated?

by Dr. Ray Nims

Until fairly recently, it has been common practice to authenticate human cell cultures using phenotypic status (e.g., receptor or protein expression) and animal species of origin testing. This level of authentication is better than none, but it is not sufficient to unambiguously identify a human cell culture. The result has been that we are still hearing about cases of misidentified human cells being used for biomedical research.

There are now methods available that are capable of rapidly and unambiguously identifying human cell lines, tissues, and cell preparations to the individual level. The recent demonstration of the potential utility of molecular technologies such as short tandem repeat (STR) and single nucleotide polymorphism (SNP) profiling for cell authentication has provided the impetus for development of a new standardized method for human cell authentication.

To this end, an ATCC Standards Development Organization workgroup with international representation has spent the past two years developing a consensus standard for the Authentication of Human Cell Lines through STR Profiling. The forthcoming Standard will provide guidance on the use of STR profiling for authenticating human cells, tissue, and cell lines. It will contain methodological detail on the preparation and extraction of the DNA, guidance on the appropriate numbers and types of loci to be evaluated and on interpretation and quality control of the results. Associated with the standard itself will be the establishment of a public STR profile database which will be administered and maintained by the National Center for Biotechnology Information (NCBI). The database primarily will contain STR profiles of commonly used cell lines.

                                            STR Profiling of Hela Cells

 An announcement that the Standard is now available for public 45-day review, comment, and vote was published in the October 22, 2010 issue of the ANSI newsletter Standards Action.

The benefits of the Standard will depend on the degree to which it is adopted and followed in the biomedical research and development and biopharmaceutical  communities. Taking a broader view, it is hoped that funding agencies and journals will begin to use such authentication standards as important considerations for funding or publishing research employing human cells. The quality and validity of funded and published research should benefit greatly as a result of the reduction in frequency of use of misidentified human cells.

The deadline for comments is December 6, 2010. There is still time to review the draft Standard and to voice your opinions and concerns.

What cell line is this anyway?

By Dr. Ray Nims

For about as long as scientists have been using cell cultures in biomedical research, there have been cases of cell line misidentification. This has been especially true for continuous cell lines, with the increased probability over time of mislabeling or cross-contamination. The primary cross-contaminant historically has been HeLa, a human cervical carcinoma cell which, given the opportunity, could outgrow most other cells in culture. More recently, the use of feeder cells for the propagation of human stem cells, and the use of xenografting for the propagation of human tumor cells, has provided additional opportunities for cell line cross-contamination and misidentification.


In the past, confirmation of cell line species of origin has been the main approach for authenticating cell lines. This was done initially by karyotyping or by immunological techniques, but more recently it has been done through the technique of isoenzyme analysis. An example of an isoenzyme analysis is shown below for Peptidase B and Aspartate Aminotransferase.  These agarose gels show a positive control, a negative control (this is the band that does not line up with the others), the test article and a standard extract.  These gels confirmed the identity of the test article as mouse derived, as expected. 

Isoenzyme analysis has the advantage that it is rapid, not very technically demanding, and may be used not only to confirm species of origin but also to detect the presence of a cross-contaminating cell if the latter is present in the culture at 10% or greater (Nims et al., Sensitivity of Isoenzyme Analysis for the Detection of Interspecies Cell Line Cross-Contamination. In Vitro Cell. Dev. Biol.-Animal 34:35-39, 1998). In fact, isoenzyme analysis is currently the primary method employed within the biopharmaceutical industry for cell line authentication in satisfaction of 1993 Points to Consider and ICH Q5D guidance.

Recent advances in molecular diagnostic techniques have made possible the authentication of human cell lines to the individual level. DNA fingerprinting technologies have matured to the point that some of them, especially single nucleotide polymorphism (SNP) typing and single tandem repeat (STR) profiling, are now considered to be viable options for standardizing human cell authentication (see ATCC SDO newsletter article, page 5. For both human and animal cells, DNA fingerprinting provides a means of determining authenticity to the individual level. However, the primary drawback is that the fingerprinting techniques as routinely performed will be less or not at all useful for detecting interspecies cocultivations or cross-contaminations. For this purpose, it may be necessary to retain isoenzyme analysis as part of the authentication armament even when the molecular technologies become the definitive authentication practices for human and animal cell lines.